Journal: Data in Brief

Article Title: Data on binding of L-tryptophan and bovine serum albumin by novel gold nanoparticles capped with amphiphilic sulfonatomethylated calixresorcinarenes

doi: 10.1016/j.dib.2019.104241

Figure Lengend Snippet: Specifications table

Article Snippet: How data was acquired , Spectrophotometry: Perkin Elmer Instruments TEM: Libra 120 IR: Bruker Vector 22 Fluorimetry: Varian Cary Eclipse DLS: Zetasizer Nano instrument.

Techniques: Microscopy, Spectrophotometry, Plasmid Preparation, Synthesized, Binding Assay

Journal: The Journal of Biological Chemistry

Article Title: Oviductosome-Sperm Membrane Interaction in Cargo Delivery

doi: 10.1074/jbc.M114.633156

Figure Lengend Snippet: Electron micrographs of mouse OVS. Oviductosomes, exosomes, and microvesicles, were isolated using differential ultracentrifugation. A, overview image of negatively stained samples with uranyl acetate was acquired by transmission electron microscopy. Bar, 100 nm. Immunogold-labeled samples were stained with uranyl acetate, a heavy metal stain that binds to lipids and proteins, prior to imaging with transmission electron microscopy. B, microvesicles (**) from IgG control ranged in sizes from 0.1 to 1 μm in diameter and exosomes (*) are < 100 nm, and showed no immunogold particles. C, immunogold labeling of PMCA4 (arrows) showed ultra-small gold particles on microvesicles (**). D, enlarged inset of C. Images were acquired with a Zeiss. LIBRA 120 TEM at 120 kV. Bar, 200 nm.

Article Snippet: LIBRA 120 TEM at 120kV equipped with a Gatan Ultrascan 1000 CCD camera.

Techniques: Isolation, Staining, Transmission Assay, Electron Microscopy, Labeling, Imaging

Journal: The Journal of Biological Chemistry

Article Title: Oviductosome-Sperm Membrane Interaction in Cargo Delivery

doi: 10.1074/jbc.M114.633156

Figure Lengend Snippet: FM4–64FX-labeled OVS and immunogold labeling in OVS fused with WT sperm membrane provide evidence for involvement of a fusogenic mechanism in the transfer of PMCA4. A, fusion of a FM4–64FX-labeled microvesicle (green arrow) with the sperm membrane over the acrosome was seen in SR-SIM images. Sperm nuclei were stained with DRAQ5 (blue). In C, sperm incubated in labeled OVS acquire the label and PMCA4 (green signal) on the plasma membrane overlying the acrosomal cap and the midpiece where the green signal for transferred PMCA4 merges with the red OVS to give yellow (yellow arrows). Note that endogenous PMCA4 does not localize to the midpiece. The green staining on the head and the proximal principal piece (blue arrow) is the location of endogenous PMCA4. Representative images from a total of n = ∼12 and 18 sperm, respectively. Images were acquired with a 63× Plan-Apochromatic oil immersion objective (numerical aperture of 1.4) B and D, TEM analysis demonstrates and confirms fusion (green arrow). Inset shows transfer of gold particles (PMCA4, yellow arrow) from OVS to the sperm membrane over the acrosome. Control IgG shows the alignment of OVS along the sperm membrane with the absence of immunogold labeling (D). Representative images from a total of n = ∼30 in each group. Images were acquired with a Zeiss. LIBRA 120 TEM at 120kV equipped with a Gatan Ultrascan 1000 CCD camera.

Article Snippet: LIBRA 120 TEM at 120kV equipped with a Gatan Ultrascan 1000 CCD camera.

Techniques: Labeling, Staining, Incubation

Journal: The Journal of Biological Chemistry

Article Title: Oviductosome-Sperm Membrane Interaction in Cargo Delivery

doi: 10.1074/jbc.M114.633156

Figure Lengend Snippet: FM4–64FX-labeled OVS and immunogold labeling in OVS fused with WT sperm membrane provide evidence for involvement of a fusogenic mechanism in the transfer of PMCA4. A, fusion of a FM4–64FX-labeled microvesicle (green arrow) with the sperm membrane over the acrosome was seen in SR-SIM images. Sperm nuclei were stained with DRAQ5 (blue). In C, sperm incubated in labeled OVS acquire the label and PMCA4 (green signal) on the plasma membrane overlying the acrosomal cap and the midpiece where the green signal for transferred PMCA4 merges with the red OVS to give yellow (yellow arrows). Note that endogenous PMCA4 does not localize to the midpiece. The green staining on the head and the proximal principal piece (blue arrow) is the location of endogenous PMCA4. Representative images from a total of n = ∼12 and 18 sperm, respectively. Images were acquired with a 63× Plan-Apochromatic oil immersion objective (numerical aperture of 1.4) B and D, TEM analysis demonstrates and confirms fusion (green arrow). Inset shows transfer of gold particles (PMCA4, yellow arrow) from OVS to the sperm membrane over the acrosome. Control IgG shows the alignment of OVS along the sperm membrane with the absence of immunogold labeling (D). Representative images from a total of n = ∼30 in each group. Images were acquired with a Zeiss. LIBRA 120 TEM at 120kV equipped with a Gatan Ultrascan 1000 CCD camera.

Article Snippet: LIBRA 120 TEM at 120kV equipped with a Gatan Ultrascan 1000 CCD camera.

Techniques: Labeling, Staining, Incubation